Review



tkov3 grna library  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Addgene inc tkov3 grna library
    (a) Raw readcounts before sequencing depth normalization per gRNA for all 71,090 gRNAs in the <t>TKOv3</t> library at T0. Shown is a FASN query screen as a representative example. (b) Total number of raw readcounts per screen at T0 for each query and control screen. Overall, 356 unique T0 samples from the 363 screens existed in the initial dataset, since several control screens that were performed in different media conditions shared a common T0. (c) Number of gRNAs with readcounts before read-depth normalization below 40 for each screen. (d) Mean number of gRNAs flagged on each chromosome compared to the number of gRNAs targeting genes on those chromosomes. (e) Number of screens a gRNA is flagged in. Only the 2092 gRNAs that are flagged in at least one T0 sample (screen) are shown. (f) Total number of raw readcounts per screen at T18 (screen endpoint) for each query and control screen. Overall, 1167 unique end and intermediate time point samples (technical triplicates) were sequenced from the 363 unique screens. (g, h) Raw (g) and normalized log2 (h) readcounts per gRNA for all 71,090 gRNAs in the TKOv3 library at T18 (endpoint). Shown is a FASN query screen as a representative example. (i) Normalized log2 readcount T0 hybrid versus T18 (endpoint) comparison of all 71,090 gRNAs in the TKOv3 library. Shown is a FASN query screen as a representative example. (j) T18 over T0 hybrid foldchange (lfc) of the FASN screen. (k) Area under the precision-recall curve (AUPRC) distinguishing the Hart et al. core essential genes from non-essential genes as a basic per-screen quality control statistic.
    Tkov3 Grna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tkov3 grna library/product/Addgene inc
    Average 94 stars, based on 81 article reviews
    tkov3 grna library - by Bioz Stars, 2026-04
    94/100 stars

    Images

    1) Product Images from "Quantitative analysis of genetic interactions in human cells from genome-wide CRISPR-Cas9 screens"

    Article Title: Quantitative analysis of genetic interactions in human cells from genome-wide CRISPR-Cas9 screens

    Journal: bioRxiv

    doi: 10.1101/2025.06.30.662330

    (a) Raw readcounts before sequencing depth normalization per gRNA for all 71,090 gRNAs in the TKOv3 library at T0. Shown is a FASN query screen as a representative example. (b) Total number of raw readcounts per screen at T0 for each query and control screen. Overall, 356 unique T0 samples from the 363 screens existed in the initial dataset, since several control screens that were performed in different media conditions shared a common T0. (c) Number of gRNAs with readcounts before read-depth normalization below 40 for each screen. (d) Mean number of gRNAs flagged on each chromosome compared to the number of gRNAs targeting genes on those chromosomes. (e) Number of screens a gRNA is flagged in. Only the 2092 gRNAs that are flagged in at least one T0 sample (screen) are shown. (f) Total number of raw readcounts per screen at T18 (screen endpoint) for each query and control screen. Overall, 1167 unique end and intermediate time point samples (technical triplicates) were sequenced from the 363 unique screens. (g, h) Raw (g) and normalized log2 (h) readcounts per gRNA for all 71,090 gRNAs in the TKOv3 library at T18 (endpoint). Shown is a FASN query screen as a representative example. (i) Normalized log2 readcount T0 hybrid versus T18 (endpoint) comparison of all 71,090 gRNAs in the TKOv3 library. Shown is a FASN query screen as a representative example. (j) T18 over T0 hybrid foldchange (lfc) of the FASN screen. (k) Area under the precision-recall curve (AUPRC) distinguishing the Hart et al. core essential genes from non-essential genes as a basic per-screen quality control statistic.
    Figure Legend Snippet: (a) Raw readcounts before sequencing depth normalization per gRNA for all 71,090 gRNAs in the TKOv3 library at T0. Shown is a FASN query screen as a representative example. (b) Total number of raw readcounts per screen at T0 for each query and control screen. Overall, 356 unique T0 samples from the 363 screens existed in the initial dataset, since several control screens that were performed in different media conditions shared a common T0. (c) Number of gRNAs with readcounts before read-depth normalization below 40 for each screen. (d) Mean number of gRNAs flagged on each chromosome compared to the number of gRNAs targeting genes on those chromosomes. (e) Number of screens a gRNA is flagged in. Only the 2092 gRNAs that are flagged in at least one T0 sample (screen) are shown. (f) Total number of raw readcounts per screen at T18 (screen endpoint) for each query and control screen. Overall, 1167 unique end and intermediate time point samples (technical triplicates) were sequenced from the 363 unique screens. (g, h) Raw (g) and normalized log2 (h) readcounts per gRNA for all 71,090 gRNAs in the TKOv3 library at T18 (endpoint). Shown is a FASN query screen as a representative example. (i) Normalized log2 readcount T0 hybrid versus T18 (endpoint) comparison of all 71,090 gRNAs in the TKOv3 library. Shown is a FASN query screen as a representative example. (j) T18 over T0 hybrid foldchange (lfc) of the FASN screen. (k) Area under the precision-recall curve (AUPRC) distinguishing the Hart et al. core essential genes from non-essential genes as a basic per-screen quality control statistic.

    Techniques Used: Sequencing, Control, Comparison



    Similar Products

    tkov3 grna library  (Addgene inc)
    94
    Addgene inc tkov3 grna library
    (a) Raw readcounts before sequencing depth normalization per gRNA for all 71,090 gRNAs in the <t>TKOv3</t> library at T0. Shown is a FASN query screen as a representative example. (b) Total number of raw readcounts per screen at T0 for each query and control screen. Overall, 356 unique T0 samples from the 363 screens existed in the initial dataset, since several control screens that were performed in different media conditions shared a common T0. (c) Number of gRNAs with readcounts before read-depth normalization below 40 for each screen. (d) Mean number of gRNAs flagged on each chromosome compared to the number of gRNAs targeting genes on those chromosomes. (e) Number of screens a gRNA is flagged in. Only the 2092 gRNAs that are flagged in at least one T0 sample (screen) are shown. (f) Total number of raw readcounts per screen at T18 (screen endpoint) for each query and control screen. Overall, 1167 unique end and intermediate time point samples (technical triplicates) were sequenced from the 363 unique screens. (g, h) Raw (g) and normalized log2 (h) readcounts per gRNA for all 71,090 gRNAs in the TKOv3 library at T18 (endpoint). Shown is a FASN query screen as a representative example. (i) Normalized log2 readcount T0 hybrid versus T18 (endpoint) comparison of all 71,090 gRNAs in the TKOv3 library. Shown is a FASN query screen as a representative example. (j) T18 over T0 hybrid foldchange (lfc) of the FASN screen. (k) Area under the precision-recall curve (AUPRC) distinguishing the Hart et al. core essential genes from non-essential genes as a basic per-screen quality control statistic.
    Tkov3 Grna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tkov3 grna library/product/Addgene inc
    Average 94 stars, based on 1 article reviews
    tkov3 grna library - by Bioz Stars, 2026-04
    94/100 stars
    		  Buy from Supplier
    toronto knockout version 3 0 tkov3 guide rna guide rna grna library  (Addgene inc)
    94
    Addgene inc toronto knockout version 3 0 tkov3 guide rna guide rna grna library
    (a) Raw readcounts before sequencing depth normalization per gRNA for all 71,090 gRNAs in the <t>TKOv3</t> library at T0. Shown is a FASN query screen as a representative example. (b) Total number of raw readcounts per screen at T0 for each query and control screen. Overall, 356 unique T0 samples from the 363 screens existed in the initial dataset, since several control screens that were performed in different media conditions shared a common T0. (c) Number of gRNAs with readcounts before read-depth normalization below 40 for each screen. (d) Mean number of gRNAs flagged on each chromosome compared to the number of gRNAs targeting genes on those chromosomes. (e) Number of screens a gRNA is flagged in. Only the 2092 gRNAs that are flagged in at least one T0 sample (screen) are shown. (f) Total number of raw readcounts per screen at T18 (screen endpoint) for each query and control screen. Overall, 1167 unique end and intermediate time point samples (technical triplicates) were sequenced from the 363 unique screens. (g, h) Raw (g) and normalized log2 (h) readcounts per gRNA for all 71,090 gRNAs in the TKOv3 library at T18 (endpoint). Shown is a FASN query screen as a representative example. (i) Normalized log2 readcount T0 hybrid versus T18 (endpoint) comparison of all 71,090 gRNAs in the TKOv3 library. Shown is a FASN query screen as a representative example. (j) T18 over T0 hybrid foldchange (lfc) of the FASN screen. (k) Area under the precision-recall curve (AUPRC) distinguishing the Hart et al. core essential genes from non-essential genes as a basic per-screen quality control statistic.
    Toronto Knockout Version 3 0 Tkov3 Guide Rna Guide Rna Grna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/toronto knockout version 3 0 tkov3 guide rna guide rna grna library/product/Addgene inc
    Average 94 stars, based on 1 article reviews
    toronto knockout version 3 0 tkov3 guide rna guide rna grna library - by Bioz Stars, 2026-04
    94/100 stars
    		  Buy from Supplier
    tkov3 sgrna library  (Addgene inc)
    93
    Addgene inc tkov3 sgrna library
    (a) Raw readcounts before sequencing depth normalization per gRNA for all 71,090 gRNAs in the <t>TKOv3</t> library at T0. Shown is a FASN query screen as a representative example. (b) Total number of raw readcounts per screen at T0 for each query and control screen. Overall, 356 unique T0 samples from the 363 screens existed in the initial dataset, since several control screens that were performed in different media conditions shared a common T0. (c) Number of gRNAs with readcounts before read-depth normalization below 40 for each screen. (d) Mean number of gRNAs flagged on each chromosome compared to the number of gRNAs targeting genes on those chromosomes. (e) Number of screens a gRNA is flagged in. Only the 2092 gRNAs that are flagged in at least one T0 sample (screen) are shown. (f) Total number of raw readcounts per screen at T18 (screen endpoint) for each query and control screen. Overall, 1167 unique end and intermediate time point samples (technical triplicates) were sequenced from the 363 unique screens. (g, h) Raw (g) and normalized log2 (h) readcounts per gRNA for all 71,090 gRNAs in the TKOv3 library at T18 (endpoint). Shown is a FASN query screen as a representative example. (i) Normalized log2 readcount T0 hybrid versus T18 (endpoint) comparison of all 71,090 gRNAs in the TKOv3 library. Shown is a FASN query screen as a representative example. (j) T18 over T0 hybrid foldchange (lfc) of the FASN screen. (k) Area under the precision-recall curve (AUPRC) distinguishing the Hart et al. core essential genes from non-essential genes as a basic per-screen quality control statistic.
    Tkov3 Sgrna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tkov3 sgrna library/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    tkov3 sgrna library - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    toronto knockout version 3 0 tkov3 guide rna guide rna grna library 15  (Addgene inc)
    94
    Addgene inc toronto knockout version 3 0 tkov3 guide rna guide rna grna library 15
    (a) Raw readcounts before sequencing depth normalization per gRNA for all 71,090 gRNAs in the <t>TKOv3</t> library at T0. Shown is a FASN query screen as a representative example. (b) Total number of raw readcounts per screen at T0 for each query and control screen. Overall, 356 unique T0 samples from the 363 screens existed in the initial dataset, since several control screens that were performed in different media conditions shared a common T0. (c) Number of gRNAs with readcounts before read-depth normalization below 40 for each screen. (d) Mean number of gRNAs flagged on each chromosome compared to the number of gRNAs targeting genes on those chromosomes. (e) Number of screens a gRNA is flagged in. Only the 2092 gRNAs that are flagged in at least one T0 sample (screen) are shown. (f) Total number of raw readcounts per screen at T18 (screen endpoint) for each query and control screen. Overall, 1167 unique end and intermediate time point samples (technical triplicates) were sequenced from the 363 unique screens. (g, h) Raw (g) and normalized log2 (h) readcounts per gRNA for all 71,090 gRNAs in the TKOv3 library at T18 (endpoint). Shown is a FASN query screen as a representative example. (i) Normalized log2 readcount T0 hybrid versus T18 (endpoint) comparison of all 71,090 gRNAs in the TKOv3 library. Shown is a FASN query screen as a representative example. (j) T18 over T0 hybrid foldchange (lfc) of the FASN screen. (k) Area under the precision-recall curve (AUPRC) distinguishing the Hart et al. core essential genes from non-essential genes as a basic per-screen quality control statistic.
    Toronto Knockout Version 3 0 Tkov3 Guide Rna Guide Rna Grna Library 15, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/toronto knockout version 3 0 tkov3 guide rna guide rna grna library 15/product/Addgene inc
    Average 94 stars, based on 1 article reviews
    toronto knockout version 3 0 tkov3 guide rna guide rna grna library 15 - by Bioz Stars, 2026-04
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) Raw readcounts before sequencing depth normalization per gRNA for all 71,090 gRNAs in the TKOv3 library at T0. Shown is a FASN query screen as a representative example. (b) Total number of raw readcounts per screen at T0 for each query and control screen. Overall, 356 unique T0 samples from the 363 screens existed in the initial dataset, since several control screens that were performed in different media conditions shared a common T0. (c) Number of gRNAs with readcounts before read-depth normalization below 40 for each screen. (d) Mean number of gRNAs flagged on each chromosome compared to the number of gRNAs targeting genes on those chromosomes. (e) Number of screens a gRNA is flagged in. Only the 2092 gRNAs that are flagged in at least one T0 sample (screen) are shown. (f) Total number of raw readcounts per screen at T18 (screen endpoint) for each query and control screen. Overall, 1167 unique end and intermediate time point samples (technical triplicates) were sequenced from the 363 unique screens. (g, h) Raw (g) and normalized log2 (h) readcounts per gRNA for all 71,090 gRNAs in the TKOv3 library at T18 (endpoint). Shown is a FASN query screen as a representative example. (i) Normalized log2 readcount T0 hybrid versus T18 (endpoint) comparison of all 71,090 gRNAs in the TKOv3 library. Shown is a FASN query screen as a representative example. (j) T18 over T0 hybrid foldchange (lfc) of the FASN screen. (k) Area under the precision-recall curve (AUPRC) distinguishing the Hart et al. core essential genes from non-essential genes as a basic per-screen quality control statistic.

    Journal: bioRxiv

    Article Title: Quantitative analysis of genetic interactions in human cells from genome-wide CRISPR-Cas9 screens

    doi: 10.1101/2025.06.30.662330

    Figure Lengend Snippet: (a) Raw readcounts before sequencing depth normalization per gRNA for all 71,090 gRNAs in the TKOv3 library at T0. Shown is a FASN query screen as a representative example. (b) Total number of raw readcounts per screen at T0 for each query and control screen. Overall, 356 unique T0 samples from the 363 screens existed in the initial dataset, since several control screens that were performed in different media conditions shared a common T0. (c) Number of gRNAs with readcounts before read-depth normalization below 40 for each screen. (d) Mean number of gRNAs flagged on each chromosome compared to the number of gRNAs targeting genes on those chromosomes. (e) Number of screens a gRNA is flagged in. Only the 2092 gRNAs that are flagged in at least one T0 sample (screen) are shown. (f) Total number of raw readcounts per screen at T18 (screen endpoint) for each query and control screen. Overall, 1167 unique end and intermediate time point samples (technical triplicates) were sequenced from the 363 unique screens. (g, h) Raw (g) and normalized log2 (h) readcounts per gRNA for all 71,090 gRNAs in the TKOv3 library at T18 (endpoint). Shown is a FASN query screen as a representative example. (i) Normalized log2 readcount T0 hybrid versus T18 (endpoint) comparison of all 71,090 gRNAs in the TKOv3 library. Shown is a FASN query screen as a representative example. (j) T18 over T0 hybrid foldchange (lfc) of the FASN screen. (k) Area under the precision-recall curve (AUPRC) distinguishing the Hart et al. core essential genes from non-essential genes as a basic per-screen quality control statistic.

    Article Snippet: In brief, CRISPR library virus production was performed in HEK293T cells using the lentiviral lentiCRISPRv2 vector containing the TKOv3 gRNA library (Addgene #90294) .

    Techniques: Sequencing, Control, Comparison